By B. Jones, P. T. Lynch, J. B. Power, M. R. Davey (auth.), Paul T. Lynch, Michael R. Davey (eds.)
Electrical Manipulation of Cells presents an authoritative and updated evaluation of the sphere, masking the entire significant options in one resource. The ebook gains huge assurance that levels from the mechanisms of motion of exterior electric fields on organic fabric to the ways that electric stimuli are hired to control cells. Bringing jointly the paintings of best overseas specialists, the ebook covers membrane breakdown, gene supply, electroporation, electrostimulation, phone circulation, hybridoma creation, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation.
for every subject, the authors speak about the relevance of the method of the present cutting-edge of biotechnology. Electrical Manipulationof Cells is an unrivaled resource of knowledge for someone interested in the manipulation of cells, rather biotechnologists, cellphone biology, microbiologists, biophysicists and plant scientists. For researchers, the e-book presents technical fabric that ccan be hired of their personal paintings. scholars will achieve thorough appreciation of the functions of this crucial technique.
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Additional info for Electrical Manipulation of Cells
Other mechanisms such as electrical field stimulated endocytosis for DNA uptake are possible. [Adapted from Xie et al. (1990)]. help to design simpler and more reliable methods for applications in biomedical, agricultural, and ecological research. ACKNOWLEDGMENTS The author thanks past and present colleagues for their contributions to the project and C. J. Gross for help preparing the manuscript. S. Office of Naval Research. , and Zimmermann, U. (1979). Reversible electric breakdown of lipid bilayer membranes: A charge-pulse relaxation study.
A. (1968). Effects of high electric fields in microorganisms. III. Lysis of erythrocytes and protoplasts. Biochim. Biophys. Acta. 163: 37-43. Electrically Stimulated Membrane Breakdown 35 Schwan, H. P. (1983). Biophysics of the interaction of electromagnetic energy with cells and membranes. Pages 213-231. In Biological Effects and Dosimetry of Nonionizing Radiation. , Michaelson, S. , and Rindi, A. , Plenum Press, New York. Serpersu, E. , Kinosita, K. and Tsong, T. Y. (1985). Reversible and irreversible modification of membrane permeability by electric field.
TSONG and Tsang, 1977). The extent of red cell hemolysis was plotted either against the electrical field strength or against the power input (Joule heating). Cell lysis was found to correlate only with field strength, indicating that PEF-induced cell lysis was due to the effects of the electrical field and was unrelated to Joule heating of the bulk solution (Fig. 2-2). When the kinetics of ionic and molecular movement across the PEF-treated erythrocyte were monitored, it was found that the electrical stimulation did not lead to a global breakdown of the cell membranes (Kinosita and Tsang, 1977).
Electrical Manipulation of Cells by B. Jones, P. T. Lynch, J. B. Power, M. R. Davey (auth.), Paul T. Lynch, Michael R. Davey (eds.)