By E. Schnepf (auth.), Prof. Dr. W. Wiessner, Prof. D. G. Robinson, Dr. R. C. Starr (eds.)
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Extra resources for Compartments in Algal Cells and Their Interaction
After transport the chloroplasts were treated and separated as described in Fig. 5 action and offers the advantage of cells of different developmental stages within one leaf. Among the translation products from poly(A) RNA a. protein of 24,000 MW shows up with a maximum of expression at 2 h of illumination (Fig. 7), indicating that the correspondent mRNA is occurring rapidly and disappearing from the poly(A) RNA fraction. Other peculiarities visible in this figure have been considered already  .
8) we obtain again a double band at about 17,000 MW but in this case of equal intensity as well as an additional band of approximately 14,000 again more prominent than that in the homologous system. For this reason it is hardly possible to establish a precursor product relationship, since we cannot decide whether the data represent incomplete or rate-limiting processing, or whether there are indeed two proteins of similar intensity; this question can only be decided by means of proteolytic fingerprinting.
We have also recently sequenced the psbA gene in a uniparental mutant of C. reinhardii that is resistant to the herbicides DCMU and atrazine: it differs from the wild type at a single base pair, resulting in the substitution of serine by alanine in the predicted sequence of the 32,000 MW protein of photosystem II (Erickson et al. 1983b). In addition to providing another site for the correlation of the genetic and physical maps, this finding has other imp'ortant implications: it provides us with a well defmed selectable marker to attempt chloroplast transformation (see below) and establishes the molecular basis for herbicide resistance in the mutant.
Compartments in Algal Cells and Their Interaction by E. Schnepf (auth.), Prof. Dr. W. Wiessner, Prof. D. G. Robinson, Dr. R. C. Starr (eds.)