By Th. Bücher (auth.), Professor Dr. Horst Sund, Professor Dr. Volker Ullrich (eds.)
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Previous postulates on the mechanism have not taken into account such a "charge relay" system, Figure 8 shows a possible mechanism which includes the effect of Asp 168, the insertion of Arg 109 into the active center during catalysis, and the oil-water histidine proposal of Parker and Holbrook (1977) as modified by Grau et al. (1981a) and Birktoft and Banaszak (1983). 1 In Fig. 8a, the apoenzyme with the NAD in the process of binding is depicted. As the adenine finds its hydrophobic pocket, so Arg 101 is attracted to the pyrophosphate group which is the trigger for closing aown the loop.
In 1967, the Prussian guards officer still mentions in a letter to Manfred von Ardenne : "The greater the resistance I met with, all the more I attacked and all the better became my arms. )". At the bottom of his heart, Warburg remained a "staff officer in plain clothes" with all reservations against "civilians". Notwithstanding all criticism, he thought highly of the military: "Here a purer air reigns". " The fact that I was probably the only one of his pupils (see Krebs' description of his separation from Warburg ) who had never had any serious quarrel with him, may be attributed to the fact that I, too, had been trained in a Brandenburgian unit.
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Biological Oxidations by Th. Bücher (auth.), Professor Dr. Horst Sund, Professor Dr. Volker Ullrich (eds.)