By Professor Dr. Ulrich Winkler, Doz. Dr. Wolfgang Rüger, Priv.-Doz. Dr. Wilfried Wackernagel (auth.)
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Additional resources for Bacterial, Phage and Molecular Genetics: An Experimental Course
Procedures in Nucleic Acid Research, 667 p. London: Harper and Row Publ. 1966 and Vol. 2, 924 p. (1971). N. ): The Nucleic Acids. 3 Volumes. New York: Academic Press 1955-1960. : The Biochemistry of the Nucleic Acids, 396 p. London: Chapman and Hall 1972. E. ): Progress in Nucleic Acid Research and Molecular Biology. 14 Volumes. London: Academic Press 1963-1974. , MOLDAVE, K. ): Methods in Enzymology, Vol. 21. Nucleic Acids, Part D. London: Academic Press 1971. : DNA Synthesis, 399 p. San Francisco: Freeman and Co.
G. 30 (log E - log Eo)/t (min-I). (9) The optical density at A = 580 nm obtained with a suspension of non-pigmented bacteria is exclusively due to light scattering by the cells. In a certain range the number of cells per ml is proportional to the optical density of the suspension. Measurements of light scattering by bacteria is also possible at other wavelengths in the range 400 - 700 nm. 31 2. Purification and Concentration of Phage T 4 a) Dextransulfate - PEG Two-Phase Technique A crude lysate contains the phages and cellular debris plus dissolved components of the lysed host bacteria.
No. of fractions For the graphical evaluation, use the right ordinate and the diiagonal standard curve, whose slope isdeter~ined by the given s-values and the position of the maxima of activity of the reference molecules. 9 S f3-Galactosidase I I (a second maximum) 23 S The 23 S maximum of f3-galactosidase does not appear in all preparations of f3-galactosidase. : Biological Chemistry. Theory of Ultracentrifugation, see pp. 80 ff, 218 ff. New York- London: Harper & Row 1971. A. ): Handbook of Biochemistry.
Bacterial, Phage and Molecular Genetics: An Experimental Course by Professor Dr. Ulrich Winkler, Doz. Dr. Wolfgang Rüger, Priv.-Doz. Dr. Wilfried Wackernagel (auth.)